SPRY2 antibody (AA 1-315)

Catalog No. ABIN7599261

This anti-SPRY2 antibody is a Rabbit Polyclonal antibody detecting SPRY2 in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for SPRY2 antibody (AA 1-315) (ABIN7599261)

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SPRY2 Antibody

Binding Specificity: AA 1-315

Purpose: Anti-Spry-2/SPRY2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Spry-2/SPRY2 Antibody Picoband® (ABIN7599261). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Spry-2/SPRY2 recombinant protein (Position: M1-T315).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Milillo, A., La Carpia, F., Costanzi, S., D'Urbano, V., Martini, M., Lanuti, P., Vischini, G., Larocca, L. M., Marchisio, M., Miscia, S., Amoroso, A., Gurrieri, F., Sangiorgi, E. A SPRY2 mutation leading to MAPK/ERK pathway inhibition is associated with an autosomal dominant form of IgA nephropathy. Europ. J. Hum. Genet. 23: 1673-1678, 2015. 2. Shim, K., Minowada, G., Coling, D. E., Martin, G. R. Sprouty2, a mouse deafness gene, regulates cell fate decisions in the auditory sensory epithelium by antagonizing FGF signaling. Dev. Cell 8: 553-564, 2005. 3. Taketomi, T., Yoshiga, D., Taniguchi, K., Kobayashi, T., Nonami, A., Kato, R., Sasaki, M., Sasaki, A., Ishibashi, H., Moriyama, M., Nakamura, K., Nishimura, J., Yoshimura, A. Loss of mammalian Sprouty2 leads to enteric neuronal hyperplasia and esophageal achalasia. Nature Neurosci. 8: 855-857, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Alternative Name: SPRY2

Background:

Synonyms: Protein sprouty homolog 2, Spry-2, SPRY2

Tissue Specificity: Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.

Background: Sprouty homolog 2 (Drosophila), also known as SPRY2, is a protein which in humans is encoded by the SPRY2 gene. It is mapped to 13q31.1. This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is inly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Molecular Weight: 35 kDa

Gene ID: 10253

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation